Journal: Reports of Biochemistry & Molecular Biology
Article Title: Vitamin D Binding Protein (DBP), Free Calcidiol, and Total Calcitriol in Adults from Northern Greece
doi: 10.61186/rbmb.12.4.652
Figure Lengend Snippet: Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by ELISA. Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.
Article Snippet: At the Clinical Chemistry Laboratory, Faculty of Biomedical Sciences, IHU, we measured free calcidiol, total calcitriol, and DBP concentrations, using commercial immunoenzymatic kits: Free 25OH Vitamin D ELISA (Immuno‐Biological Laboratories Inc., USA) for free calcidiol, 1,25(OH)2 Vitamin D ELISA (DIAsource ImmunoAssays, Belgium) for total calcitriol, and Human Vitamin DBP Immunoassay Quantikine® ELISA (China R&D Systems Ltd., China) for DBP, according to the manufacturer’s instructions.
Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay